Macrofungi are considered organisms that form large fruiting bodies above or below the ground that are visible without the aid of a microscope. These fungi include most basidiomycetes and a small number of ascomycetes. Macrofungi have different ecological roles and uses, where some are edible, medicinal, poisonous, decomposers, saprotrophs, predators, and pathogens, and they are often used for innovative biotechnological, medicinal, and ecological applications. However, comprehensive checklists and compilations on the diversity and distribution of mushrooms are lacking for South Africa, which makes regulation, conservation, and inclusion in national biodiversity initiatives difficult. In this review, we compiled a checklist of macrofungi for the first time (excluding lichens). Data were compiled based on available literature in journals, books, and fungorium records from the National Collection of Fungi. Even if the list is not complete due to numerous unreported species present in South Africa, it still represents an overview of the current knowledge of the micromycetes of South Africa. The list of names enables the assessment of gaps in collections and knowledge on the fungal biodiversity of South Africa and downstream applications such as defining the residency status of species. It provides a foundation for new names to be added in the future towards developing a list that will be as complete as possible, and that can be used by a wide audience including scientists, authorities, and the public.
