Urbanization by densification is globally increasing and endangers maintenance of urban green and associated social-ecological systems. Cultural ecosystem services play a crucial role in human well-being, especially in urban areas. We analyzed perceived importance of cultural ecosystem services provided by green space in Berlin along an urban-periurban gradient. Based on extensive pretests, we designed a standardized questionnaire and conducted 558 face-to-face interviews. B using multiple regressions and principal component analysis, we show that perceived importance of cultural ecosystem services and patterns of urban green use are affected by an urbanization gradient and associated changes in population density. Important cultural ecosystem services decreases in urban core areas with higher population density, whereas people in periurban areas with more available green spaces exhibit a greater valuation of nature. In contrast, social relations and cultural diversity had the highest importance in the urban core, while cultural heritage, education, natural awareness, recreation, and aesthetical appreciation were higher valued in the less populated periurban areas, suggesting two bundles of cultural ecosystem services.